National Repository of Grey Literature 4 records found  Search took 0.00 seconds. 
Testování antimikrobiální aktivity obalových materiálů s rostlinnými extrakty proti bakteriím rodu Enterococcus
Maňáková, Simona
This thesis deals with testing of antimicrobial activity of plant essential oils as part of packaging systems for foodpackaging against bacteria genus Enterococcus. The first literature part is focused on characteristic of genus Enterococcus and his possible apperace in food and his negative effect on human health. Next topic of literature section is targeted on using plants as producers of essential oils and their use in food industry as active compounds with antimicrobial and atifugal activity. This includes also characteristic of selected aromatic plants growing in our geographical conditions. Last part of literature section is introduction of characteristic of packaging materials and theirs big importance in food industry. Practical part of this thesis is dealing with testing of packaging materials containg essential oils on theirs surface against E. faecalis. Another part of this experiment is testing these antimicrobial packaging materials on selected foods in this case meat product following with microbiological analysis of selected bacteria.
Detekce patogenů pomocí molekulárně imprintovaných polymerů
Hutařová, Jitka
The theoretical part of this diploma thesis deals with molecularly imprinted polymers. Properties of the monomers and templates used for the polymerization are described. Part is devoted to the preparation of molecularly imprinted polymers and interactions leading to the formation of imprinted polymers. Imprinting technologies and strategies, template removal methods, detection methods, and the advantages and disadvantages of the molecular imprinting are included. Also the applications in various fields, focusing mainly on the area of imprinting proteins and pathogens are discussed in details. The experimental part deals with optimization of the preparation of a molecularly imprinted polymer based on dopamine subsequently used especially for the isolation and detection of Staphylococcus aureus and Enterococcus faecalis. The polymer was coated over a microscope slide, into the 96-well microplate and on a surface of magnetic particles. Fluorescence spectrometry and fluorescence microscopy were used for analyte detection. The developed technique was capable of isolating the target (Staphylococcus aureus and Enterococcus faecalis) at relatively low concentrations (1.103 CFU.ml-1).

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